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Astrocytes produce the antiinflammatory and neuroprotective agent hydrogen sulfide

Identifieur interne : 002119 ( Main/Exploration ); précédent : 002118; suivant : 002120

Astrocytes produce the antiinflammatory and neuroprotective agent hydrogen sulfide

Auteurs : Moonhee Lee [Canada] ; Claudia Schwab [Canada] ; SHENG YU [Canada] ; Edith Mcgeer [Canada] ; Patrick L. Mcgeer [Canada]

Source :

Neurobiology of aging [ 0197-4580 ] ; 2009.

RBID : Pascal:11-0160689

Descripteurs français

Pascal (Inist)
- Astrocyte, Antiinflammatoire, Neuroprotecteur, Cystathionine β-synthase, Microglie, Sénescence, Démence d'Alzheimer, Maladie de Parkinson, Neuroinflammation.

English descriptors

KwdEn :
- Alzheimer disease, Antiinflammatory agent, Astrocyte, Cystathionine β-synthase, Microglia, Neuroinflammation, Neuroprotective agent, Parkinson disease, Senescence.

Abstract

Hydrogen sulfide (H₂S) is an essential physiological product in brain. We investigated the expression of cystathionine-β-synthase (CBS) and cystathionine-γ-lyase (CGL), the two H₂S synthesizing enzymes, in human cell lines and in human brain. Only astrocytes were strongly immunostained for CBS. Cultured astrocytes synthesized H₂S at the rate of 15.06 μmol/g protein/h, which was 7.57 fold higher than microglial cells, 10.27 fold higher than SH-SY5Y cells and 11.32 fold higher than NT-2 cells. The H₂S synthesis in all these cell types was inhibited by the CBS inhibitor hydroxylamine, but not by the CGL inhibitor propargylglycine (PAG). Synthesis of H₂S by HUVEC cells was inhibited by PAG but not by hydroxylamine indicating that these vascular cells utilize CGL but not CBS. Inflammatory activation of microglia and astrocytes caused induction of NFκB, release of the inflammatory mediators TNFα, IL-6 and nitrite ions, down-regulation of CBS, and down-regulation of H₂S synthesis. There was no effect of such treatment on HUVEC cells. The effects were partially reversed by pretreatment of cells with the H₂S releasing agent NaSH. These data indicate that H₂S is an endogenous antiinflammatory and neuroprotective agent under the synthetic control of CBS. H₂S releasing drugs may have therapeutic potential in neurodegenerative disorders of aging such as Alzheimer disease and Parkinson disease.

Affiliations:

Canada

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Le document en format XML

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<front><div type="abstract" xml:lang="en">Hydrogen sulfide (H<sub>2</sub>
S) is an essential physiological product in brain. We investigated the expression of cystathionine-β-synthase (CBS) and cystathionine-γ-lyase (CGL), the two H<sub>2</sub>
S synthesizing enzymes, in human cell lines and in human brain. Only astrocytes were strongly immunostained for CBS. Cultured astrocytes synthesized H<sub>2</sub>
S at the rate of 15.06 μmol/g protein/h, which was 7.57 fold higher than microglial cells, 10.27 fold higher than SH-SY5Y cells and 11.32 fold higher than NT-2 cells. The H<sub>2</sub>
S synthesis in all these cell types was inhibited by the CBS inhibitor hydroxylamine, but not by the CGL inhibitor propargylglycine (PAG). Synthesis of H<sub>2</sub>
S by HUVEC cells was inhibited by PAG but not by hydroxylamine indicating that these vascular cells utilize CGL but not CBS. Inflammatory activation of microglia and astrocytes caused induction of NFκB, release of the inflammatory mediators TNFα, IL-6 and nitrite ions, down-regulation of CBS, and down-regulation of H<sub>2</sub>
S synthesis. There was no effect of such treatment on HUVEC cells. The effects were partially reversed by pretreatment of cells with the H<sub>2</sub>
S releasing agent NaSH. These data indicate that H<sub>2</sub>
S is an endogenous antiinflammatory and neuroprotective agent under the synthetic control of CBS. H<sub>2</sub>
S releasing drugs may have therapeutic potential in neurodegenerative disorders of aging such as Alzheimer disease and Parkinson disease.</div>
</front>
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<tree><country name="Canada"><noRegion><name sortKey="Lee, Moonhee" sort="Lee, Moonhee" uniqKey="Lee M" first="Moonhee" last="Lee">Moonhee Lee</name>
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<name sortKey="Sheng Yu" sort="Sheng Yu" uniqKey="Sheng Yu" last="Sheng Yu">SHENG YU</name>
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Astrocytes produce the antiinflammatory and neuroprotective agent hydrogen sulfide

Astrocytes produce the antiinflammatory and neuroprotective agent hydrogen sulfide

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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